HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that preserved HuR-RNA interactions, and HuR-bound target mRNAs were identified by cDNA array hybridization.

RNA-binding protein HuR binds to and stabilizes OIP5-AS1. Among the vast collection of lncRNAs annotated for human sequences (9837 lncRNAs, Ensembl v72) (35, 36), OIP5-AS1 was identified as the human homolog of cyrano, a lncRNA that plays a role in zebrafish development , and showed significant conservation in gene structure .

Human antigen R (HuR) is an RNA-binding protein that regulates the stability, translation, and nucleus-to-cytoplasm shuttling of its target mRNAs. While HuR is normally localized within the nucleus, it has been shown that HuR binds mRNAs in the nucleus and then escorts the mRNAs to the cytoplasm where HuR protects them from degradation. RNA‐binding protein HuR suppresses senescence through Atg7 mediated autophagy activation in diabetic intervertebral disc degeneration 1 INTRODUCTION. Low back pain is a common symptom of various medical conditions, and up to 80% of the population may 2 MATERIAL AND METHODS. All treatments on The RNA binding protein HuR determines the differential translation of autism-associated FoxP subfamily members in the developing neocortex The RNA-binding protein HuR is a negative regulator in adipogenesis Abstract. Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains Introduction. Obesity is characterized by excessive lipid storage in adipose tissue, leading to an increase of The RNA-binding protein HuR binds to elements rich in adenylate and uridylate (AU-rich elements) in target mRNAs and stabilizes them against degradation.

Genesis 45, 413-417. Crossref, Medline, Google Scholar Growing evidence shows that cancer cells use mRNA-binding proteins and miRNAs to posttranscriptionally regulate signaling pathways to adapt to harsh tumor microenvironments. In ovarian cancer, cytoplasmic accumulation of mRNA-binding protein HuR (ELAVL1) is associated with poor prognosis. In this study, we observed high HuR expression in ovarian cancer cells compared with ovarian primary cells RNA-binding proteins represent an emerging class of proteins with a role in renal dysfunction. We show that activation of the RNA-binding protein Hu antigen R (HuR) is increased in glomerulosclerosis not only in anti-Thy 1.1 nephritis model but also in varied human glomerular diseases. 2021-04-04 · RNA-binding protein HuR regulates translation of vitamin D receptor modulating rapid epithelial restitution after wounding.

The negative control RNA [poly(A) 25 RNA] does not contain HuR or poly(C) BP binding sites. Incubation of A431 lysate with labeled AR UTR RNA enriches HuR RBP; incubation with only poly(A) 25 RNA or HuR is a member of the embryonic lethal abnormal vision (ELAV) family of RNA-binding proteins .

The RNA-binding protein, HuR, associates with the HuR mRNA, but the consequences of this interaction are unknown. Here, we use human

Schumacher, Jill; Keesook Lee; Susanne Edelhoff; Robert Braun (May 1995). "Spnr, a Murine RNA-binding Protein That Is Localized to "Intracellular re-routing of prion protein prevents propagation of PrP(Sc) and delays "Long-term memory consolidation: The role of RNA-binding proteins with 0, 8, fig|393124.3.peg.2573, Listeria monocytogenes FSL N3-165, RNA-binding protein Jag, GO:0003723, cmr|LMOf2365_2843, cmr|LMOh7858_3116, Human antigen R (HuR) is a widely expressed RNA binding protein that interacts with specific AU-rich domains in target mRNAs and exerts av A Gormand · 2014 · Citerat av 18 — cAMP-response element-binding protein (CREB) is required for the and the mRNA expression of adipogenic transcription factors Cebpa, Human antigen R (HuR) is an RNA-binding protein that regulates the stability, translation, and nucleus-to-cytoplasm shuttling of its target mRNAs. While HuR is normally localized within the nucleus, it has been shown that HuR binds mRNAs in the nucleus and then escorts the mRNAs to the cytoplasm where HuR protects them from degradation. ELAV-like protein 1 or HuR is a protein that in humans is encoded by the ELAVL1 gene.

Jan 10, 2020 HuR (also known as Elavl1) is a universally expressed RBP. It recognizes and binds to the AU-rich elements (ARE) in 3′UTR of its target

Poly(C) Binding Proteins (CP1 and CP2). These RNA-binding proteins regulate mRNA stability (HuR) and mRNA turnover and translation (CP1 and CP2). The negative control RNA [poly(A) 25 RNA] does not contain HuR or poly(C) BP binding sites. Incubation of A431 lysate with labeled AR UTR RNA enriches HuR RBP; incubation with only poly(A) 25 RNA or HuR is a member of the embryonic lethal abnormal vision (ELAV) family of RNA-binding proteins . It can shuttle between the nucleus and the cytoplasm, and the stabilization of ARE-mRNA by HuR is believed to be linked to its localization in the cytoplasm (12,13). The ubiquitously expressed mRNA‐binding protein (mRBP) HuR (ELAVL1) is a postulated stress‐relevant protein that binds to adenylate uridylate (AU)‐rich sites within regulatory mRNA targets and regulates their expression via post‐transcriptional mechanisms .

Hu proteins possess three RNA-recognition motifs through which they bind with high affinity and specificity to target Posttranscriptional gene regulation by RNA-binding proteins, such as HuR (elavl1), fine-tune gene expression in T cells, leading to powerful effects on immune responses.
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Among the vast collection of lncRNAs annotated for human sequences (9837 lncRNAs, Ensembl v72) (35, 36), OIP5-AS1 was identified as the human homolog of cyrano, a lncRNA that plays a role in zebrafish development , and showed significant conservation in gene structure . Poly(C) Binding Proteins (CP1 and CP2). These RNA-binding proteins regulate mRNA stability (HuR) and mRNA turnover and translation (CP1 and CP2).

2020-01-10 · Human antigen R (HuR) is an RNA binding protein that promotes mRNA stability. Here the authors show that HuR represses adipogenesis in white and brown adipose tissue by stabilizing Insig1 and ELAV-like protein 1 or HuR is a protein that in humans is encoded by the ELAVL1 gene. The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements.
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RNA Binding Proteins: New Concepts in Gene Regulation: 16: Sandberg, Kathryn, Mulroney,: Amazon.se: Books.

Accession Number, NP_001185765.1. Gene ID (Entrez), 100526737. Name, RNA-binding protein 14 Protein. Purity, Greater than 90% as determined by av MG till startsidan Sök — Hur många som insjuknar i Welanders distala myopati varje år varierar av (kodar för) TIA1 cytotoxic granule associated RNA binding protein.

Role of RNA binding protein HuR in ductal carcinoma in situ of the breast. Mira Heinonen, Annabrita Hemmes, Kaisa Salmenkivi, Kotb Abdelmohsen, Suvi-Tuuli

RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha.

CDC6, a central regulator of DNA replication, is regulated by HuR. In this study, we investigated the role of HuR in colorectal cancer tumorigenesis and oxaliplatin (L-OHP) resistance, as well as the underlying mechanisms involving CDC6. We detected increased HuR and CDC6 expression Coordinating such RNA regulons is often the responsibility of regulatory RBPs that have key roles in immunity like the polypyrimide track protein 1 (PTBP1), embryonic lethal abnormal vision like protein 1 (ELAVL1, also known as HuR), or the members of the zinc finger protein 36 family (ZFP36, ZFP36L1 and ZFP36L2; also known as TTP or Tis11-family of proteins). The RNA-binding protein HuR associates with the p53 mRNA, as reported previously, and with the NPM mRNA, computationally predicted to be a target of HuR. Here, we show that HuR binds the NPM and p53 3′-untranslated regions and stabilizes these mRNAs in polyamine-depleted intestinal epithelial cells. Albeit the suppression of mRNA translation may derive from eIF3a binding to the 5′-UTRs of target mRNAs, how eIF3a may accelerate mRNA translation remains unknown. In this study, we show that eIF3a up-regulates translation of Chk1 but not Chk2 mRNA by interacting with HuR, which binds directly to the 3′-UTR of Chk1 mRNA.